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ENZYMES

Enzymes are proteins that catalyze 

( i.e. speed up ) biochemical reaction

and are not changed during the     reaction.

The life of living organisms is a reflection of what is    going on in their bodies.

Metabolism is the set of biochemical reactions that occur in living organisms in order to maintain life.

These processes allow organisms to grow and reproduce, maintain their structure and respond to their environment.

Anabolism includes the biochemical processes in which larger molecules are synthesize.

Catabolism include the biochemical reactions in which are broken down.

 

So, energy is release din the catabolism and energy is consumed in anabolism.

In this way, the biochemical reaction are actually energy transfers.

Enzymes are crucial to metabolism because they act as biocatalyst and speed up and regulate the metabolic pathway.


The molecules at which enzymes act are called Substrate

Enzymes converts them into different molecules called Product


Activation Energy

ØAll chemical reaction require activation energy.
ØIt is define as the minimum amount of energy which is require to start a biological reaction.
ØThe need for the activation energy acts as barrier to the beginning of reaction.
ØEnzymes lower such barrier by decreasing the requirement of activation energy.

ØThus in the presence of enzymes, reaction proceed at faster rate.  


> Enzymes lower the activation energy in several 
    ways:

1: They may alter the shape of substrate and reduce
    requirement of energy for this change.

2: Some enzymes do so by disrupting the charge
    distribution of activation energy.

3: Enzymes may also lower activation energy by
    bringing substrate in the correct orientation to
    react. 


Categories of enzymes


1 : Intracellular enzymes : The enzymes work  inside the 

      cell. E.g. enzymes of glycolysis working  in the

      cytoplasm.

2: Extracellular enzymes: The enzymes work outside

     the cell e.g. pepsin enzymes working in the

      stomach cavity.








 Apoptosis & Necrosis

Apoptosis and Necrosis are two

Phenomenon of cell death.

                                                                                                             In an adult

                                                                                                               Human, 50 to

                                                                                                               70 Billion cells

                                                                                                                Die each day by

                                                                                                              Apoptosis.

Apoptosis

ØApoptosis is one of the main type of programmed cell death. During apoptosis, cell shrinks and becomes rounded due to the breakdown of cytoskeleton by enzymes.

ØIts chromatin undergoes condensation and nuclear envelop breaks. In this way nucleus spreads in the form of several discrete chromatin bodies.

ØCell membrane makes buds known as blebs. Blebs break off from the cell and are now called apoptotic bodies, which then phagocytosed by other cells.

> Apoptosis can occur when a cell is damaged or undergoes stress   conditions.

> Apoptosis removes the damaged cell, preventing it from getting further nutrients, or to prevent the spread of infection.

> Apoptosis also gives advantages during development. For example during the formation of fingers, the cells between them undergo apoptosis and the digits separate.


Necrosis


ØNecrosis is the accidental death of cells and living tissues.

ØNecrosis is less sequential than apoptosis.
ØThere are many causes of necrosis including injury, infection, cancer etc.

ØNecrosis may occur when a cell is given hypoxic ( with less oxygen ) environment.
ØDuring necrosis, there is a release of special enzymes from lysosomes.
ØLysosomal enzymes break cellular components and may also be released outside cell to break surrounding cells.
ØCells that die by necrosis may also released harmful chemicals that damage other cells.

 



Significance of Meiosis

The significance of meiosis for reproduction and inheritance was described in 1890 by German biologist August Weismann.

He pointed out that meiosis was necessary not only  to maintain the number of chromosome sin the next generation but also to produce variation in next generation.

Maintain of the  chromosomes number in next generation  

ØMeiosis is essential for sexual reproduction. In Human, diploid gamete-mother or germ line cell undergo meiosis to produce haploid gametes.
ØMale and female gametes unite to form diploid zygote, which undergoes repeated mitosis and develops into a new diploid human.

ØPlants life cycle shows alternation of generation.  The cell of sporophyte generation undergo meiosis to produce haploid spores, which grow into haploid gametophyte generation. Gametophyte generation produce haploid gametes through mitosis. The gametes combine to produce diploid zygote. Zygote undergo repeated mitosis to become diploid sporophyte generation.  

Produce of variation
  in next generation

ØThe  chromosomes pairs of each parent undergo crossing over during meiosis. So daughter cells . i.e. gametes have genetic variation.
ØWhen gametes fuse and form zygote, its genetic make is different from both parents.
ØThus meiosis allows a species to bring variations in the next generation.

ØBeneficial help organisms to adapt to the changes in environment.


Errors in Meiosis


ØDuring anaphase I, chromosomes separate and go to opposite poles while during anaphase II, sister chromosomre separate. It is called disjunction.
ØSometime the separation is not normal and it is called non-disjunction. This result in the production of gametes which have either more or less than the normal number of chromosomes.

Øif such gametes fuses with normal gametes, it result abnormal chromosomes number in next generation , for example 47 0r 45 chromosomes in human.